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  • Title: Label-fracture of cell surfaces by replica staining.
    Author: Forsman CA, Pinto da Silva P.
    Journal: J Histochem Cytochem; 1988 Nov; 36(11):1413-8. PubMed ID: 2459187.
    Abstract:
    We introduce replica-staining label-fracture, a method for the cytochemical mapping of membrane surfaces. This method is a corollary of the rationale of label-fracture (Pinto da Silva and Kan, 1984: J Cell Biol 99:1156). After freeze-fracture the exoplasmic halves of the membrane remain attached to the replica. We show that cytochemical labeling of cell surfaces can be performed by direct post-fracture staining of freeze-fracture replicas. This new variant of label-fracture leads to miniaturization of labeling procedures and allows standardization of labeling conditions and simultaneous processing of different specimens.
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