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  • Title: [Decalcification in formic acid after fixation in zinc-ethanol-formaldehyde does not preclude identification of neuronal and glial markers].
    Author: Kolos EA, Korzhevskiĭ DE.
    Journal: Morfologiia; 2013; 144(4):76-9. PubMed ID: 24592706.
    Abstract:
    The aim of this study was to determine the possibility of immunohistochemical demonstration of various tissue antigens in paraffin sections of zinc-ethanol-formaldehyde-fixed spinal cord, surrounded by the vertebral tissues and adjacent muscles, after decalcification in formic acid, in adult (n = 5) and newborn (n = 2) Wistar rats. In the present work, the antibodies used were against: glial fibrillary acidic protein, neurofilaments, neuronal specific nuclear protein, enzyme tyrosine hydroxylase, protein of synaptic vesicles--synaptophysin and the intermediate filament protein--vimentin. Combination of fixation in zinc-ethanol-formaldehyde and decalcification in 21% formic acid solution was shown to provide an optimal preservation of the antigens studied.
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