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  • Title: Demonstration of immunochemical identity between the synaptic vesicle proteins synaptin and synaptophysin/p38.
    Author: Gaardsvoll H, Obendorf D, Winkler H, Bock E.
    Journal: FEBS Lett; 1988 Dec 19; 242(1):117-20. PubMed ID: 2462508.
    Abstract:
    The synaptic vesicle proteins synaptin and synaptophysin/p38 were shown to be immunochemically identical. Western immunoblot analysis of Triton X-100 extracts from rat brain showed that polyclonal polyspecific anti-synaptin antibodies and monoclonal antibody SY38 against synaptophysin both reacted with a band of 38 kDa. In two-dimensional immunoblots of chromaffin granule membranes from bovine adrenal medulla anti-synaptin and anti-synaptophysin antibodies also recognized the same component. Finally, in a Western immunoblotting experiment SY38 reacted with an immuno-isolated synaptin antigen.
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