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  • Title: Sodium influxes in renal epithelial LLC-PK1/Cl4 cells monitored by 23Na NMR.
    Author: Jans AW, Willem R, Kellenbach ER, Kinne RK.
    Journal: Magn Reson Med; 1988 Jul; 7(3):292-9. PubMed ID: 2462662.
    Abstract:
    23Na NMR experiments with a suspension of the renal epithelial cell line, LLC-PK1/Cl4, in the presence of the shift reagent dysprosium polyphosphate showed that the intracellular sodium was only NMR visible for 64 +/- 4%. Intracellular sodium content was found to be 30.6 +/- 1.2 mM (25 degrees C). Examination of the sodium influx during recovery from intracellular acidification showed that sodium is transported not only by Na+/H+ exchange but also by sodium-D-glucose cotransport with a stoichiometry of 1:1.
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