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  • Title: The self-association of the giant hemoglobin from the earthworm, Lumbricus terrestris.
    Author: Riggs AF, Riggs CK.
    Journal: Biochim Biophys Acta; 2014 Jun; 1844(6):1071-5. PubMed ID: 24631544.
    Abstract:
    BACKGROUND: The crystallographic structure of the gigantic hemoglobin (erythrocruorin) of the annelid worm, Lumbricus terrestris, provides a molar mass of 3.6MDa for the hexagonal bilayer structure. Prior to this determination, some light-scattering and ultracentrifugal measurements indicated higher masses: 4.1-4.4MDa. Values of 3.6MDa were attributed to dissociation or subunit loss. However, early electron microscopy of the giant hemoglobin from a related annelid, Eumenia crassa by Öster Levin, showed that the hexagonal bilayer molecules were present mostly as oligomers; few were monomeric. METHODS: Measurements by light-scattering of solutions of Lumbricus hemoglobin resolved by size-exclusion chromatography have been used to determine the weight-average molar mass of self-associating proteins. The X-ray structure has been re-examined. RESULTS: Our measurements show that both 3.6MDa monomers and self-association products are present as a mixture. Analysis of the X-ray structure indicates several different kinds of monomer-monomer interactions. CONCLUSIONS: We propose that the measured masses of Lumbricus hemoglobin as high as 4.4MDa, result from oligomerization. These masses would result from the presence of an array of oligomers of various sizes together with monomers of 3.6MDa. Furthermore, several different kinds of monomer-monomer interactions are clearly evident in the X-ray structure as well as in solution. GENERAL SIGNIFICANCE: The results demonstrate that self-association of monomers of the hemoglobin of Lumbricus terrestris explains the high molar masses of 4.1-4.4MDa previously observed.
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