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  • Title: Study of metalloproteins using continuous wave electron paramagnetic resonance (EPR).
    Author: Gambarelli S, Maurel V.
    Journal: Methods Mol Biol; 2014; 1122():139-51. PubMed ID: 24639258.
    Abstract:
    Electron paramagnetic resonance (EPR) is an invaluable tool when studying systems with paramagnetic centers. It is a sensitive spectroscopic method, which can be used with dilute samples in aqueous buffer solutions. Here, we describe the basic procedure for recording an X-band EPR spectrum of a metalloprotein sample at low temperature. We also discuss basic optimization techniques to provide spectra with a high signal to noise ratio and minimum distortion.
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