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  • Title: [Two-codon mutagenesis of alpha-amylase gene of Bacillus amyloliquefaciens].
    Author: Smirnova NA, Sorokin AV, Laptev DA, Veĭko VP, Kozlov IuI.
    Journal: Mol Biol (Mosk); 1988; 22(5):1265-71. PubMed ID: 2464738.
    Abstract:
    The oligonucleotide encoding Bam HI recognition site having the structure pCGGGATC had been inserted into the recognition sites MspI of the B. amyloliquefaciens alpha-amylase gene, which was cloned in pTG29B plasmid. The alpha-amylase gene had no BamHI sites before mutagenesis. The set of pNSBamHI plasmids with BamHI site at four different positions was obtained. It was shown that all the mutant alpha-amylases possess different specific activities. One of the mutant proteins possesses reduced thermostability. The mutant alpha-amylases can be used for further experiments on protein-engineering of liquefying-type alpha-amylases.
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