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  • Title: A modified Brown and Hopps stain for identification of gram-positive and gram-negative microorganisms in glycol methacrylate-embedded tissues.
    Author: Schwartz DA, Munger R, Lazzaro B, Lumb G.
    Journal: Arch Pathol Lab Med; 1989 Feb; 113(2):181-3. PubMed ID: 2464978.
    Abstract:
    Plastic embedding is a recently developed technique that has been shown to be superior to conventional paraffin embedding in the histopathologic identification of microorganisms in tissue specimens. This report describes a modification of the Brown and Hopps stain for the differentiation of gram-positive and gram-negative microorganisms in 3-micron-thick plastic sections. This technique is easily performed and has a rapid turnaround time. This staining method permits a more precise histopathologic diagnosis of infectious agents than is possible in paraffin-embedded tissues.
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