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  • Title: Two-dimensional gel electrophoresis to separate large RNA molecules such as messenger RNAs.
    Author: Ikemura T.
    Journal: Nucleic Acids Symp Ser; 1988; (19):57-60. PubMed ID: 2465540.
    Abstract:
    This paper describes a two-dimensional gel electrophoresis method for separating large RNA molecules such as messenger RNAs. RNAs were at first separated on a polyacrylamide plus agarose composite gel and subjected to a second dimension electrophoresis on a polyacrylamide gel containing urea. This method is illustrated by analyses of poly(A)+ yeast RNAs. About 80 discrete spots were detected on the gel, when RNAs from 1000 to 3500 nucleotides in size were examined.
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