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  • Title: Role of channels in the fusion of vesicles with a planar bilayer.
    Author: Woodbury DJ, Hall JE.
    Journal: Biophys J; 1988 Dec; 54(6):1053-63. PubMed ID: 2466492.
    Abstract:
    Fluorescence microscopy combined with electrical conductance measurements were used to assess fusion of phospholipid vesicles with a planar bilayer. Large unilamellar vesicles (0.5-3 microns diam.) filled with the fluorescent dye, calcein, were made both with or without porin channels. Vesicle-bilayer fusion was induced by increasing the osmolarity of the solution on the side of the bilayer to which the vesicles were added. Fusion was detected optically by the fluorescent flash due to release of vesicular contents. Although both porin-containing and porin-free vesicles give the same kind of flash upon content release, the conditions necessary to induce release are very different. Only 4% of the porin-free vesicles fuse (release their contents) when subjected to 3 M urea. However, the same conditions induce 53% of the porin-containing vesicles to fuse and most of these fusions occur at a lower osmolarity ([urea] less than 400 mM). Thus channels greatly enhance fusion in this model system. A physical model based on the postulate that fusion is induced by an increase in surface tension, predicts that three conditions are necessary for fusion in this system: (a) an open channel in the vesicle membrane, (b) an osmotic gradient across the bilayer, and (c) the vesicle in contact with the planar membrane. These are the conditions that experimentally produce fusion in the model system.
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