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  • Title: Immunostaining of tissue sections previously stained with hematoxylin and eosin.
    Author: Paul E, Cochran AJ.
    Journal: Dis Markers; 1988; 6(4):231-6. PubMed ID: 2466600.
    Abstract:
    S-100 protein was demonstrated in 28/31 melanoma tissue sections that had been previously stained by hematoxylin and eosin, including 17 from 19 primary melanomas and 11 from 12 metastases. Additional H & E stained sections from 11 metastatic and 13 primary melanomas were reacted with the monoclonal antibody NKI/C-3 and all stained positively. This approach is of value when additional sections are not available or when deeper cuts through a block do not contain tumor. The superimposition of the immunostaining does not reduce the record and diagnostic value of the double-stained sections.
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