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  • Title: One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).
    Author: Brunelle JL, Green R.
    Journal: Methods Enzymol; 2014; 541():151-9. PubMed ID: 24674069.
    Abstract:
    This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.
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