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  • Title: Plant genome engineering in full bloom.
    Author: Lozano-Juste J, Cutler SR.
    Journal: Trends Plant Sci; 2014 May; 19(5):284-7. PubMed ID: 24674878.
    Abstract:
    The recent development of tools for precise editing of user-specified sequences is rapidly changing the landscape for plant genetics and biotechnology. It is now possible to target mutations and regulatory proteins to specific sites in a genome using zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), or the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Here we provide an update of recent developments in CRISPR/Cas9 technology and highlight online resources that will help biologists adopt new genome-editing tools.
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