These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The heterogeneity of lipopolysaccharides from Citrobacter 023 serotype.
    Author: Katzenellenbogen E.
    Journal: Arch Immunol Ther Exp (Warsz); 1988; 36(2):119-28. PubMed ID: 2467634.
    Abstract:
    Chemical studies on carbohydrate part of lipopolysaccharides isolated from Citrobacter 87/57 and Citrobacter 1556 strains (serotype 023) were carried out. Lipopolysaccharides (LPS) were hydrolyzed with 1% acetic acid and the carbohydrate material was fractionated on Sephadex G-50 and Bio-Gel P-4 columns. All fractions obtained were subjected to chemical analysis by colorimetric methods and by gas-liquid chromatography. It was found that O-specific polysaccharide from LPS 87/57 is composed of mannose and N-acetylgalactosamine in the molar ratio of 3:1. Both lipopolysaccharides contain unsubstituted core oligosaccharides (built of heptose, glucose and galactose in molar ratio of 3:3:1) as well as core oligosaccharides substituted with shorter (LPS 87/57 and LPS 1556) and longer (LPS 87/57) O-specific oligosaccharides. Strain 87/57 is of S (smooth) type and strain 1556 lacking O-specific polysaccharide is of SR (semi-rough) type. The data of Sephadex G-100 chromatography showed that O-antigen from Citrobacter 87/57 is heterogeneous; it contains polysaccharide chains of different length but the same composition. The heterogeneity of Citrobacter 023 lipopolysaccharides has been also detected by SDS-polyacrylamide gel electrophoresis.
    [Abstract] [Full Text] [Related] [New Search]