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  • Title: Topochemistry of environmentally friendly pretreatments to enhance enzymatic hydrolysis of sugar cane bagasse to fermentable sugar.
    Author: Mou H, Heikkilä E, Fardim P.
    Journal: J Agric Food Chem; 2014 Apr 23; 62(16):3619-25. PubMed ID: 24689355.
    Abstract:
    In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis. The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments. Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment. Fiber morphology after pretreatments was more favorable for enzyme hydrolysis as well. However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
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