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  • Title: In vivo labeling of myelin lipids and proteolipid protein with [3H]myristate, [14C]linoleate, and [14C]linolenate.
    Author: Bürgisser P, Matthieu JM.
    Journal: Neurochem Res; 1989 Jan; 14(1):91-6. PubMed ID: 2469026.
    Abstract:
    To investigate the incorporation of essential fatty acids into myelin components, 24-day-old rabbits were injected intracerebrally with [14C]linoleate, [14C]linolenate, or [3H]myristate for comparison. Animals were killed 22 hr later and myelin was isolated. [3H]myristate labeled all myelin lipids including monogalactosyl diglyceride, with the exception of sulfatides. With 14C-essential fatty acids, only glycerophospholipids were efficiently labeled and their specific activities were in the following decreasing orders: PC greater than PI greater than PE greater than PS with [14C]linoleate, and PE greater than PC greater than PI = PS with [14C]linolenate. Among myelin proteins, PLP and DM-20 were labeled with all 3 precursors. PLP was purified from myelin labeled with 14C-essential fatty acids. The label was then cleaved from the protein by alkaline methanolysis and was identified as a dienoic [( 14C]linoleate) or a tetraenoic [( 14C]linolenate) fatty acid. MBP was not labeled with [3H]myristate, but was slightly labeled with both 14C-essential fatty acids. The signification of the latter result is discussed.
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