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  • Title: Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers on single pudendal motoneurons in Onuf's nucleus of the cat: a light microscope study combining the triple immunocytochemical staining technique with the retrograde HRP-tracing method.
    Author: Tashiro T, Satoda T, Matsushima R, Mizuno N.
    Journal: Brain Res; 1989 Mar 06; 481(2):392-8. PubMed ID: 2470468.
    Abstract:
    Convergence of serotonin (5-HT)-, enkephalin (ENK)-, and substance P (SP)-like immunoreactive (LI) afferent fibers on single pudendal motoneurons within Onuf's nucleus was demonstrated: pudendal motoneurons were retrogradely labeled with horseradish peroxidase applied to the pudendal nerve. Subsequently, 5-HT-LI fibers were stained by the immunoperoxidase method, and then ENK- and SP-LI fibers were stained by the double immunofluorescence method.
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