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  • Title: [Validation of a liquid chromatography method for rifampicin determination in human plasma].
    Author: Moreno-Exebio L, Grande-Ortiz M.
    Journal: Rev Peru Med Exp Salud Publica; 2014; 31(1):56-61. PubMed ID: 24718527.
    Abstract:
    OBJECTIVES: To validate the high-performance liquid chromatography method (HPLC) for rifampicin (RFP) determination in human plasma. MATERIALS AND METHODS: A HPLC method for RFP determination in plasma was developed. The separation was performed by reversed-phase chromatography with C18 column and a mobile phase composed of a mixture of acetonitrile and monobasic potassium phosphate buffer solution 0.05 M (38:62 v/v) at 335 nm in which standard rifampicin quinone (RFP-QN) was used. RESULTS: The retention times of RFP and RFP-QN were 7.81 and 12.26 minutes, respectively. The trial was linear from 0.5 to 250 ug/mL. The evaluated parameters of precision, accuracy, selectivity, linearity, and recovery complied with the established international standards for validation of bioanalytical methods. CONCLUSIONS: The developed HPLC method is simple, specific, sensitive, selective and linear for a wide range of RFP concentrations in plasma.
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