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  • Title: Interference of the carbohydrate moiety in coomassie brilliant blue R-250 protein staining.
    Author: Osset M, Piñol M, Fallon MJ, de Llorens R, Cuchillo CM.
    Journal: Electrophoresis; 1989 Apr; 10(4):271-3. PubMed ID: 2472958.
    Abstract:
    The binding of Coomassie Brilliant Blue R-250 to several species of bovine pancreatic ribonuclease is affected by the presence of a carbohydrate moiety in the enzyme molecule. Enzymic deglycosylation of several chromatographic fractions of ribonuclease, which have different degrees of glycosylation, results in increased staining by Coomassie Brilliant Blue R-250. Ovalbumin and other glycoproteins tested show similar behavior. The results indicate that carbohydrate moieties may represent a common hindrance to the binding of Coomassie Brilliant Blue dyes to glycoproteins.
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