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  • Title: Application of Namalva interferon-alpha monoclonal antibodies for purification and enzyme immunoassay of interferon-alpha.
    Author: Notani J, Kaizu T, Mutoh S, Otsuka K, Kusunoki C, Yamada H, Nagayoshi A, Suzuki S, Niwa M, Ueda I.
    Journal: J Immunoassay; 1989; 10(2-3):257-76. PubMed ID: 2473094.
    Abstract:
    Namalva (or Namalwa) interferon (IFN)-alpha was partially purified using a combination of conventional methods and modified acid-ethanol extraction. Four mouse monoclonal antibodies against Namalva IFN-alpha were prepared by hybridoma technology after immunization with Namalva IFN-alpha thus purified. Three of these monoclonal antibodies recognized the same or a similar epitope on Namalva IFN-alpha. One of these antibodies was paired with the fourth recognizing a different epitope and used respectively as enzyme-conjugated antibody and solid-phase antibody in our one step enzyme immunoassay (EIA) for IFN-alpha. This assay is simple and was able to detect as little as 5 pg of IFN-alpha in 100 microliters of sample in the short time of 5 hr. There was a good correlation between the EIA and bioassay. The use of one of the monoclonal antibodies as an immunoadsorbant to purify Namalva IFN-alpha is also described.
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