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  • Title: Dual channel sensitive detection of hsa-miR-21 based on rolling circle amplification and quantum dots tagging.
    Author: Wangt DC, Hu LH, Zhou YH, Huang YT, Li X, Zhu JJ.
    Journal: J Biomed Nanotechnol; 2014 Apr; 10(4):615-21. PubMed ID: 24734513.
    Abstract:
    An isothermal, highly sensitive and specific assay for the detection of hsa-miR-21 with the integration of QDs tagging and rolling circle amplification was offered. In addition, a dual channel strategy for miRNA detection was proposed: anodic stripping voltammetry (ASV) and fluorescent method were both performed for the final Cd2+ signal readout. The designed strategy exhibited good specificity to hsa-miR-21 and presented comparable detection results by detection methods.
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