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  • Title: [Isolation and partial characterization of poly-(a)+mRNA of soybean grown in the presence of gibberellic acid].
    Author: Dzhokhadze DI, Mamulashvili NA, Lomidze NV, Nazarova LA.
    Journal: Ukr Biokhim Zh (1978); 1989; 61(3):107-11. PubMed ID: 2473562.
    Abstract:
    Poly(A)+mRNA was prepared by chromatography on the oligo (dT)-cellulose column from total RNA of epicotyls of soybean grown on the medium with gibberellic acid. Poly(A)+mRNA was prepared by the same method from control plants. Total RNA, poly-A and poly(A)+mRNAs were analyzed by electrophoresis on 1.5% agarose gel under denaturing conditions. The poly(A)+mRNA distribution was shown to be of polydispersional character, cDNA was synthesized by reverse transcription. Basing on cDNA-poly(A)+mRNA hybridization a conclusion is drawn that gibberellic acid may activate new types of mRNAs and, probably, increases the synthesis of some poly(A)+mRNA populations.
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