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  • Title: Indoxyl-tetranitro blue tetrazolium method for detection of alkaline phosphatase in immunohistochemistry.
    Author: Brenan M, Bath ML.
    Journal: J Histochem Cytochem; 1989 Aug; 37(8):1299-301. PubMed ID: 2474024.
    Abstract:
    A sensitive method for detection of alkaline phosphatase in immunohistochemistry, using lymphoid cells, has been optimized. The conditions for staining are 0.23 mM 5-bromo-4-chloro-indoxyl phosphate, 0.55 mM tetranitro blue tetrazolium, 2.0 mM levamisole, 5.0 mM sodium azide, 10.0 mM magnesium chloride, and 0.15 mM 1-methoxyphenazine methosulfate dissolved in 100 mM Tris-HCl buffer, pH 9.5.
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