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  • Title: Extraction, characterization and antioxidant activities of polysaccharides from E. corneum gigeriae galli.
    Author: Xiong Q, Li X, Zhou R, Hao H, Li S, Jing Y, Zhu C, Zhang Q, Shi Y.
    Journal: Carbohydr Polym; 2014 Aug 08; 108():247-56. PubMed ID: 24751271.
    Abstract:
    In the present study, optimization of enzyme-assisted extraction, characterization and antioxidant activities in vitro of polysaccharides from Endothelium corneum gigeriae galli (PEGG) were investigated. It was found that the optimum extraction conditions were determined as follows: extraction temperature 87.0°C, extraction time 177.0 min, enzyme concentration 1.65%, enzymatic hydrolysis time 141.0 min, liquid-to-solid ratio 20, enzymatic hydrolysis temperature 55°C and enzymatic hydrolysis pH 3.6. Under these conditions, the experimental yield of polysaccharides was 5.08%. In addition, PEGG had a relatively high sulfate radical content. PEGG was composed of rhamnose, fucose, mannose, glucose and galactose, with molar percentages of 13.1, 4.5, 72.8 and 9.6%, respectively. The average molecular weight was 83 kDa. And there were infrared characteristic absorption peaks of polysaccharides in the FT-IR spectroscopy of PEGG. For antioxidant activities in vitro, PEGG showed possessed strong hydroxyl radical scavenging, Fe(2+) chelating and lipid peroxidation inhibitory activities.
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