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  • Title: Orcein-alcian blue staining: a new technique for demonstrating acid mucins in gastrointestinal epithelium.
    Author: Singh R, Gorton AW.
    Journal: J Clin Pathol; 1989 Aug; 42(8):881-4. PubMed ID: 2475531.
    Abstract:
    Orcein-alcian blue staining, a new method for the simultaneous demonstration of sulphated and sialomucins in gastrointestinal epithelium was compared with the standard high iron diamine-alcian blue technique. Sections were oxidised with potassium permanganate and decolourised in oxalic acid. They were stained with orcein for four hours, differentiated for a few seconds in acid alcohol, and then counterstained with alcian blue for half to one minute. There was a good correlation of results between the two methods. Orcein-alcian blue is a safer, cheaper, and quicker method than high iron diamine-alcian blue which can be safely introduced into routine laboratories for the study of acid mucins in the gastrointestinal diseases.
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