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  • Title: High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor.
    Author: St-Pierre F, Marshall JD, Yang Y, Gong Y, Schnitzer MJ, Lin MZ.
    Journal: Nat Neurosci; 2014 Jun; 17(6):884-9. PubMed ID: 24755780.
    Abstract:
    Accurate optical reporting of electrical activity in genetically defined neuronal populations is a long-standing goal in neuroscience. We developed Accelerated Sensor of Action Potentials 1 (ASAP1), a voltage sensor design in which a circularly permuted green fluorescent protein is inserted in an extracellular loop of a voltage-sensing domain, rendering fluorescence responsive to membrane potential. ASAP1 demonstrated on and off kinetics of ∼ 2 ms, reliably detected single action potentials and subthreshold potential changes, and tracked trains of action potential waveforms up to 200 Hz in single trials. With a favorable combination of brightness, dynamic range and speed, ASAP1 enables continuous monitoring of membrane potential in neurons at kilohertz frame rates using standard epifluorescence microscopy.
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