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  • Title: [Study on the optimum combinations of plant hormone in callus culture of Thermopsis lanceolata].
    Author: Zheng XF, Wang XD, Zhang CQ, Wang EJ.
    Journal: Zhong Yao Cai; 2013 Oct; 36(10):1555-9. PubMed ID: 24761661.
    Abstract:
    OBJECTIVE: To explore the effects of different hormone combinations on callus induction of Thermopsis lanceolata. METHODS: The effects of different hormone concentrations and ratio on callus induction and subculture multiplication of Thermopsis lanceolata were investigated by orthogonal design methods. RESULTS: The optimal medium of callus induction for hypocotyls explants was MS + 2, 4-D (0.5 mg/L) + BA (1.0 mg/L) + NAA (1.0 mg/L) + IAA (0.5 mg/L). The best medium of callus subculture multiplication for hypocotyl explants was MS with 2,4-D (0.1 mg/L) + BA (0.1 mg/L) + NAA (0.2 mg/L) + IAA (0.5 mg/L) . In the process of callus subculture multiplication, the effect extent of four kinds of hormones for hypocotyl explants was BA > 2,4-D > IAA > NAA. The growth curve of callus culture optimal hormone combination was "S" curve. CONCLUSION: The rapid callus culture system for Thermopsis lanceolata is established for the first time. It provides a new way for protecting and utilizing Thermopsis lanceolata species resources through the modern biotechnology.
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