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  • Title: Isolation of human transcribed sequences from human-rodent somatic cell hybrids.
    Author: Liu P, Legerski R, Siciliano MJ.
    Journal: Science; 1989 Nov 10; 246(4931):813-5. PubMed ID: 2479099.
    Abstract:
    A method was developed for selectively isolating genes from localized regions of the human genome that are contained in interspecific hybrid cells. Complementary human DNA was prepared from a human-rodent somatic cell hybrid that contained less than 1% human DNA, by using consensus 5' intron splice sequences as primers. These primers would select immature, unspliced messenger RNA (still retaining species-specific repeat sequences) as templates. Screening a derived complementary DNA library for human repeat sequences resulted in the isolation of human clones at the anticipated frequency with characteristics expected of exons of transcribed human genes--single copy sequences that hybridized to discrete bands on Northern (RNA) blots.
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