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  • Title: [Preparation of a suspension of isolated hepatocytes using the Soviet drug collalitin].
    Author: Toshchakov VIu, Danilov MA, Shestakova NK, Kozlovskaia TI, Kulikova EIu.
    Journal: Vopr Med Khim; 1989; 35(4):134-7. PubMed ID: 2479173.
    Abstract:
    Isolation of individual hepatocytes suspension from rats was carried out using trypsin, collagenase and collalytine; maximal yield of the viable hepatocytes was obtained when 0.05% solution of collagenase was used. Trypsin was ineffective in production of viable cells, while collalytine from pig pancreas exhibited the satisfactory results at concentration of 0.15-0.20%.
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