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  • Title: MB-02 cells undergo fetal to adult globin gene switching in response to erythropoietin.
    Author: Perrine SP, Miller BA, Morgan DA.
    Journal: Biochem Biophys Res Commun; 1989 Oct 31; 164(2):857-62. PubMed ID: 2479377.
    Abstract:
    The recently described MB-02 human cell line requires Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) for continuous growth and terminally differentiates into enucleate, hemoglobinized cells in response to erythropoietin. Here, analysis of globin production now demonstrates that uninduced MB-02 cells produce alpha globin and the fetal globin chains G gamma and A gamma in a ratio of 1:3. Addition of erythropoietin results in de novo synthesis of beta globin chains and a marked increase in total Hb/cell. Thus, the MB-02 cell line partially recapitulates the fetal to adult globin switch that occurs during erythroid and human fetal development and provides a new clonal human erythroid progenitor system for investigating the biochemical and molecular events involved in globin gene switching.
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