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  • Title: Fibreoptic bronchoscopy: an assessment of immediate cytological diagnosis using methylene blue stain.
    Author: Titley I, Davidson RN, Turner E, Hicks ER, Cooke NT, Landells WN, Levene MM.
    Journal: Respir Med; 1989 Jan; 83(1):37-41. PubMed ID: 2479962.
    Abstract:
    A method of examining cytological material during fibreoptic bronchoscopy using a methylene blue (MB) stain was assessed in 164 consecutive fibreoptic bronchoscopies where cytology specimens were taken. The MB method provided an immediate positive diagnosis in 86% of bronchoscopically visible tumours. Subsequent histology provided a positive diagnosis in 69%, conventional brush cytology in 81% and trap cytology in 77%. The MB method produced no false positive diagnosis of malignancy and the tumour cell type identified by MB stain agreed with the histological cell type in 72% of cases. This technique is considered to be sufficiently specific to provide a method of controlling the quality of specimens taken at bronchoscopy, for further analysis in the laboratory.
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