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  • Title: Nuclear factor I represses the reverse-oriented transcription from the adenovirus type 5 DNA terminus.
    Author: Matsumoto K, Nagata K, Yamanaka K, Hanaoka F, Ui M.
    Journal: Biochem Biophys Res Commun; 1989 Nov 15; 164(3):1212-9. PubMed ID: 2480114.
    Abstract:
    The promoter activity of a cloned inverted terminal repeat (ITR) of human adenovirus (Ad) type 5 was found to be oriented in the opposite direction with respect to that of DNA replication in a cell-free transcription system using HeLa nuclear extracts. The major transcript was initiated outside the Ad sequence about 30 nucleotides downstream from the putative TATA-box located between nucleotide positions 9-18 of Ad 5 left terminus. Competitive transcription experiments using double-stranded oligonucleotides and isolated nuclear factor I revealed that nuclear factor I and its cognate binding site located upstream of the putative TATA-box are involved in negative regulation of the transcription from ITR promoter.
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