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  • Title: A patch-clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes. Pressure-activated channels are localized in the inner membrane.
    Author: Berrier C, Coulombe A, Houssin C, Ghazi A.
    Journal: FEBS Lett; 1989 Dec 18; 259(1):27-32. PubMed ID: 2480919.
    Abstract:
    Inner and outer membranes of Escherichia coli and contact zones were isolated and fused separately with giant liposomes amenable to patch-clamp recording. Different types of large pressure-activated channels were localized in the inner membrane fraction which also contained smaller, pressure-insensitive channels. The outer membrane contained pressure-insensitive channels with large conductances and long opening and closing times which are likely to be porins. Large channels were also observed in contact zones.
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