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  • Title: Agrobacterium-mediated transformation of barley (Hordeum vulgare L.).
    Author: Ismagul A, Mazonka I, Callegari C, Eliby S.
    Journal: Methods Mol Biol; 2014; 1145():203-11. PubMed ID: 24816670.
    Abstract:
    Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes. This chapter reports a refined protocol for the systematic high-throughput transformation of the advanced Australian spring barley breeding line WI4330.
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