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  • Title: An extremely sensitive aptasensor based on interfacial energy transfer between QDS SAMs and GO.
    Author: Sun X, Liu B, Yang C, Li C.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2014 Oct 15; 131():288-93. PubMed ID: 24835931.
    Abstract:
    In this work we designed a fluorescent self-assemblied multilayers, with thrombin aptamers and ssDNA as aptamer fixed onto the outermost layer, respectively. This multilayers can effectively sense biomolecules by interfacial florescence resonance energy transfer from multilayers to graphene oxide. High fluorescence quenching efficiency of graphene oxide and self-assemblied membrane' concentration results in good sensitivity for biosensing. A new interfacial sensing method with extremely high sensitivity for thrombin and DNA sequence was established, and the detection limit for thrombin and DNA was 16.2pM and 72.6 fM, respectively.
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