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Title: Portable evanescent wave fiber biosensor for highly sensitive detection of Shigella. Author: Xiao R, Rong Z, Long F, Liu Q. Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2014 Nov 11; 132():1-5. PubMed ID: 24836193. Abstract: A portable evanescent wave fiber biosensor was developed to achieve the rapid and highly sensitive detection of Shigella. In this study, a DNA probe was covalently immobilized onto fiber-optic biosensors that can hybridize with a fluorescently labeled complementary DNA. The sensitivity of detection for synthesized oligonucleotides can reach 10(-10) M. The surface of the sensor can be regenerated with 0.5% sodium dodecyl sulfate solution (pH 1.9) for over 30 times without significant deterioration of performance. The total analysis time for a single sample, including the time for measurement and surface regeneration, was less than 6 min. We employed real-time polymerase chain reaction (PCR) and compared the results of both methods to investigate the actual Shigella DNA detection capability of the fiber-optic biosensor. The fiber-optic biosensor could detect as low as 10(2) colony-forming unit/mL Shigella. This finding was comparable with that by real-time PCR, which suggests that this method is a potential alternative to existing detection methods.[Abstract] [Full Text] [Related] [New Search]