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  • Title: Electrochemical immunosensor for detection of topoisomerase based on graphene-gold nanocomposites.
    Author: Zhong GX, Wang P, Fu FH, Weng SH, Chen W, Li SG, Liu AL, Wu ZY, Zhu X, Lin XH, Lin JH, Xia XH.
    Journal: Talanta; 2014 Jul; 125():439-45. PubMed ID: 24840469.
    Abstract:
    A facile electrochemical immunosensor based on graphene-three dimensional nanostructure gold nanocomposites (G-3D Au) using simple and rapid one-step electrochemical co-reduction technique was developed for sensitive detection of topoisomerase. The resultant G-3D Au nanocomposites were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy, and then were used as a substrate for construction of the "sandwich-type" immunosensor. Amperometric current-time curve was employed to monitor the immunoreaction on the protein modified electrode. The proposed method could respond to topoisomerase with a linear calibration range from 0.5 ng mL(-1) to 50 ng mL(-1) with a detection limit of 10 pg mL(-1). This new biosensor exhibited a fast amperometric response, high sensitivity and selectivity, and was successfully used in determining the topoisomerase which was added in human serum with a relative standard deviation (n=5)<5%. The immunosensor served as a significant step toward the practical application of the immunosensor in clinical diagnosis and prognosis monitor.
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