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  • Title: [Effects of 12/15-lipoxygenase antisense oligonucleotide on peroxisome proliferator-activated receptor γ translocation in primarily cultured cortical neurons after oxygen-glucose deprivation].
    Author: Han J, Sun L, Liang H, Cheng Y.
    Journal: Zhonghua Yi Xue Za Zhi; 2014 Apr 22; 94(15):1179-83. PubMed ID: 24924720.
    Abstract:
    OBJECTIVE: To explore the effects of 12/15-lipoxygenase antisense oligonucleotide (asON-12/15-LOX) on OGD (oxygen-glucose deprivation)-induced PPARγ (peroxisome proliferator-activated receptor γ) expression and nuclear translocation in primarily cultured cortical neurons. METHODS: After a 48-h pre-treatment of 12/15-LOX antisense oligonucleotide (asON), primarily cultured cortical neurons underwent 3-hour OGD followed by a 24-hour reperfusion.Immunofluorescent staining and Western blot were used to evaluate the expressions of 12/15-LOX and PPARγ as well as the nuclear translocation of PPARγ. RESULTS: Compared with the control group, the expressions of 12/15-LOX and PPARγ whole protein were enhanced in OGD group (t = -3.72 and -6.79, P = 0.03 and 0.04). And an increase of PPARγ in nucleus (t = -4.67, P = 0.02) could be noted with a simultaneous reduction in cytosol (t = 3.40, P = 0.04) after OGD, indicating an induction of nuclear translocation by OGD. Compared with OGD group, a pre-treatment of asON-12/15-LOX dramatically attenuated OGD-induced increase in 12/15-LOX whole protein expression (t = 5.03, P = 0.02). Compared with OGD group, a pre-treatment of asON-12/15-LOX greatly reduced OGD-induced increase in PPARγ total protein expression (t = 2.83, P = 0.04) and nuclear translocation (t = 7.05, P = 0.01 for nuclear protein; t = -5.47, P = 0.01 for cytosol protein). It indicated a possible link between 12/15-LOX and PPARγ. CONCLUSION: 12/15-LOX antisense oligonucleotide suppresses the expression and nuclear translocation of PPARγ in primarily cultured cortical neurons after OGD.
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