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  • Title: Antigenicity and conformational changes of β-lactoglobulin by dynamic high pressure microfluidization combining with glycation treatment.
    Author: Zhong J, Tu Y, Liu W, Xu Y, Liu C, Dun R.
    Journal: J Dairy Sci; 2014; 97(8):4695-702. PubMed ID: 24952773.
    Abstract:
    The combined effect of previous dynamic high-pressure microfluidization treatment (40, 80, 120, and 160MPa) and subsequent glycation with galacto-oligosaccharides (GOS) on the antigenicity of β-lactoglobulin (β-LG) was investigated. The antigenicity of β-LG-GOS decreased at relatively low pressure (≤120MPa). Surface sulfhydryl group content of β-LG-GOS increased and surface hydrophobicity of β-LG-GOS decreased. Additionally, protein unfolding in β-LG-GOS samples was reflected by quenching of fluorescence intensity, the red-shift of fluorescence spectra, decreased UV absorption, and circular dichroism analysis, indicating tertiary and secondary structural changes of β-LG. The conformational changes may contribute to the alteration of antigenicity.
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