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  • Title: Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos.
    Author: Walters RD, McSwiggen DT, Goodrich JA, Kugel JF.
    Journal: PLoS One; 2014; 9(6):e101015. PubMed ID: 24968026.
    Abstract:
    The AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are unable to distinguish between the role of, for example, cJun/cJun homodimers versus cJun/cFos heterodimers. Here we used SELEX (systematic evolution of ligands by exponential enrichment) to identify and characterize DNA aptamers that are >100-fold more specific for binding cJun/cJun compared to cJun/cFos, setting the foundation to investigate the biological functions of different AP-1 dimer compositions.
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