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  • Title: Ratiometric imaging of pH probes.
    Author: Grillo-Hill BK, Webb BA, Barber DL.
    Journal: Methods Cell Biol; 2014; 123():429-48. PubMed ID: 24974041.
    Abstract:
    Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis.
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