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  • Title: Real-time PCR detection of Brucella spp. DNA in lesions and viscera of bovine carcasses.
    Author: Sola MC, da Veiga Jardim EA, de Freitas MR, de Mesquita AJ.
    Journal: J Microbiol Methods; 2014 Sep; 104():87-91. PubMed ID: 24978591.
    Abstract:
    This study reports a real-time PCR assay for the detection of Brucella spp. associated with the FTA® Elute method in lesions observed during sanitary inspections in beef slaughter. Of the total 276 samples, 78 (28.3%) tested positive and 198 (71.7%) negative for Brucella spp. The real-time PCR technique associated with the FTA® Elute method proved to be an important tool for the diagnosis, judgment about and disposal of carcasses and viscera of slaughtered animals.
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