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  • Title: Flow-based enzymatic ligation by sortase A.
    Author: Policarpo RL, Kang H, Liao X, Rabideau AE, Simon MD, Pentelute BL.
    Journal: Angew Chem Int Ed Engl; 2014 Aug 25; 53(35):9203-8. PubMed ID: 24989829.
    Abstract:
    Sortase-mediated ligation (sortagging) is a versatile, powerful strategy for protein modification. Because the sortase reaction reaches equilibrium, a large excess of polyglycine nucleophile is often employed to drive the reaction forward and suppress sortase-mediated side reactions. A flow-based sortagging platform employing immobilized sortase A within a microreactor was developed that permits efficient sortagging at low nucleophile concentrations. The platform was tested with several reaction partners and used to generate a protein bioconjugate inaccessible by solution-phase batch sortagging.
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