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  • Title: MicroRNA-27a promotes porcine myoblast proliferation by downregulating myostatin expression.
    Author: Yang T, Chen XL, Huang ZQ, Wen WX, Xu M, Chen DW, Yu B, He J, Luo JQ, Yu J, Mao XB, Zheng P.
    Journal: Animal; 2014 Nov; 8(11):1867-72. PubMed ID: 25007254.
    Abstract:
    MicroRNAs are endogenous ~22nt RNAs that negatively regulate gene expression at the posttranscriptional level via binding to the 3'-untranslated region (3'UTR) of target mRNAs. The microRNA miR-27a was reported to depress the expression of myostatin, a critical inhibitor of skeletal myogenesis, by binding to its 3'UTR in mouse. In this study, we cloned the full-length 3'UTR of porcine myostatin by rapid amplification of 3'-cDNA ends (3'-RACE) and demonstrated that the 3'UTR of porcine myostatin is targeted by miR-27a. The phenomenon that the level of myostatin inversely correlated with miR-27a was observed in fat and heart of pigs and also in proliferating porcine myoblasts. Besides, overexpression of miR-27a in porcine myoblasts promoted cell proliferation by reducing the expression of myostatin. Our data suggest that miR-27a positive regulates porcine myoblast proliferation via targeting myostatin.
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