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  • Title: An enhanced chemiluminescent enzyme immunoassay for follicle stimulating hormone.
    Author: Sankolli GM, Lynch SS, Rudd BT, Thorpe GH, Stott RA, Kricka LJ.
    Journal: J Immunoassay; 1989; 10(2-3):207-19. PubMed ID: 2501358.
    Abstract:
    An enhanced chemiluminescent enzyme immunoassay for serum follicle stimulating hormone is described which involves sequential reaction of anti-follicle stimulating hormone antibody immobilised to the inside surface of an opaque microtitre plate with sample, monoclonal anti-alpha thyroid stimulating hormone antibody, and an anti-mouse IgG - horseradish peroxidase conjungate. Bound peroxidase activity was measured using a p-hydroxycinnamic acid enhanced chemiluminescent luminol-hydrogen peroxide reaction. The assay was sensitive (detection limit 0.01 mU/well) precise (intra-assay precision 2.5-8.1%, inter-assay precision 6.7-11.9%) and results obtained with this assay and a competitive radioimmunoassay were in good agreement (correlation coefficient 0.98).
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