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  • Title: [The use of real time PCR for quantitative determination of some propionic bacteria residing on human skin].
    Author: Globa AG, Alekseev IaI, Arzumanian VG, Zaborova VA, Guridova AA.
    Journal: Biomed Khim; 2014; 60(3):372-7. PubMed ID: 25019400.
    Abstract:
    A test system has been developed for determination of propionic bacterial species residing on human skin. This system developed in the real time PCR format is applicable for quantitative determination and also detection of genomes of the following Propionibacterium species: P. acnes, P. granulosum and P. avidum. This system was used for analysis of wash samples from the skin of 17 pentathlon sportsmen and 16 students. All three species of propionic bacteria were found in all skin wash samples. However, contamination with P. acnes was two times higher in control group than in the group of pentathlon sportsmen. Razrabotana test-sistema dlia opredeleniia vidovogo sostava propionovykh bakteriĭ, obitaiushchikh na kozhe cheloveka. Dannaia sistema sozdana v formate PTsR real'nogo vremeni i pozvoliaet kolichestvenno i odnovremenno opredeliat' genomy bakteriĭ Propionibacterium vidov P. acnes, P. granulosum i P. avidum. Dlia demonstratsii raboty test-sistemy byli issledovany smyvy s uchastkov kozhi 17 sportsmenov-piatibortsev i gruppy kontrolia, sostoiashcheĭ iz 16 studentov. Bylo ustanovleno, chto u vsekh uchastnikov issledovaniia na kozhe prisutstvovali vse tri vida propionovykh bakteriĭ, prichem obsemenennost' P. acnes v gruppe kontrolia byla v 2 raza vyshe po sravneniiu s gruppoĭ sportsmenov-piatibortsev.
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