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  • Title: Sporulation of Myxococcus xanthus in liquid shake flask cultures.
    Author: Rosenbluh A, Rosenberg E.
    Journal: J Bacteriol; 1989 Aug; 171(8):4521-4. PubMed ID: 2502539.
    Abstract:
    When suspended in a liquid starvation medium, exponentially growing Myxococcus xanthus sporulated within 3 days. These myxospores were similar to spores developed within fruiting bodies, as determined by electron microscopy and the production of spore-specific protein S. This liquid sporulation system may be useful as a means of preparing large quantities of myxospores and extracellular fluid for biochemical studies, including isolation of chemical signals produced during the sporulation process.
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