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  • Title: Simultaneous quantitation and validation of triterpenoids and phytosteroids in Phaseolus angularis seeds.
    Author: Hwang JT, Oh HM, Kim MH, Jeong HJ, Lee C, Jang HJ, Lee SW, Park CS, Rho MC.
    Journal: Molecules; 2014 Jul 16; 19(7):10309-19. PubMed ID: 25033058.
    Abstract:
    A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE): oleanolic acid (1), oleanolic acid acetate (2), stigmasterol (3) and β-sitosterol (4). This method was fully validated in terms of linearity (r2 > 0.999), accuracy (98.5%-100.8%), precision (<0.92%), LOD (<0.0035 mg/mL), and LOQ (<0.0115 mg/mL). The effects of the PASE and isolated compounds 1-4 on TLR4 activation were tested in THP1-Blue cells. Among the tested substances, compound 2 showed potent inhibitory activity with an IC50 value of 3.89 ± 0.17 µM.
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