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  • Title: Unique features of monoclonal IgG2b in the cleavage reaction with pepsin.
    Author: Sumii H, Tsutsui K, Hatsushika M, Inoue H, Tanabe G, Oda T.
    Journal: Acta Med Okayama; 1989 Jun; 43(3):135-41. PubMed ID: 2504035.
    Abstract:
    Preparations of IgG2b purified from several mouse hybridoma clones were highly susceptible, compared to other subclasses, to peptic digestion under conditions usually used to prepare F (ab')2 fragments. Analyses of the digestion products revealed that no F (ab')2 was produced and that the main product was a Fab-like fragment. Demonstration of the hinge disulfides in the Fc portion clearly indicated that in IgG2b the primary peptic cleavage occurs on the NH2-terminal side of the inter-heavy chain disulfide bridge. The resulting Fab failed to bind with antigen, suggesting the importance of the CH1-hinge region in maintaining the native conformation of the antigen-binding site.
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