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  • Title: Detection of extended broad-spectrum beta-lactamases in Enterobacteriaceae in four French hospitals.
    Author: Legrand P, Fournier G, Buré A, Jarlier V, Nicolas MH, Decré D, Duval J, Philippon A.
    Journal: Eur J Clin Microbiol Infect Dis; 1989 Jun; 8(6):527-9. PubMed ID: 2504594.
    Abstract:
    In 210 strains of Enterobacteriaceae which were isolated in four hospitals and which showed reduced susceptibility to cefotaxime, high synergy was demonstrated between amoxicillin (20 micrograms) + clavulanate (10 micrograms) and cefotaxime (30 micrograms) using a simple double-disk test. Isoelectric focusing on gel and specific iodometric detection using ceftriaxone identified four extended broad-spectrum beta-lactamases (isoelectric points 7.6, 6.3, 7.0 and 5.9) produced by the strains.
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